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1 "Wanju Lee"
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Original Article
Detection of BRAFV600E Mutations in Papillary Thyroid Carcinomas by Peptide Nucleic Acid Clamp Real-Time PCR: A Comparison with Direct Sequencing
Dongjun Jeong, Yujun Jeong, Sungche Lee, Hyeran Lee, Wanju Lee, Hyungjoo Kim, Doosan Park, Soyoung Park, Wenxia Mu, Hyun-Deuk Cho, Mee-Hye Oh, Sung Soo Lee, Seung-Ha Yang, Chang-Jin Kim
Korean J Pathol. 2012;46(1):61-67.   Published online February 23, 2012
DOI: https://doi.org/10.4132/KoreanJPathol.2012.46.1.61
  • 9,759 View
  • 62 Download
  • 19 Crossref
AbstractAbstract PDF
Background

Papillary thyroid carcinoma (PTC) of the thyroid is the most common endocrine malignancy. High prevalence of an activating point mutation of BRAF gene, BRAFV600E, has been reported in PTC. We assessed the efficiency of peptide nucleic acid clamp real-time polymerase chain reaction (PNAcqPCR) for the detection of BRAFV600E mutation in PTC in comparison with direct sequencing (DS).

Methods

A total of 265 thyroid lesions including 200 PTCs, 5 follicular carcinomas, 60 benign lesions and 10 normal thyroid tissues were tested for BRAFV600E mutation by PNAcqPCR and DS.

Results

The sensitivity and accuracy of the PNAcqPCR method were both higher than those of DS for the detection of the BRAFV600E mutation. In clinical samples, 89% of PTCs harbored the BRAFV600E mutation, whereas 5 follicular carcinomas, 50 benign lesions and 10 normal thyroid tissues lacked the mutation. The mutation was associated with aggressive clinical behaviors as extrathyroid invasion (p=0.015), lymph node metastasis (p=0.002) and multiple tumor numbers (p=0.016) with statistical significance.

Conclusions

The PNAcqPCR method is efficiently applicable for the detection of the BRAFV600E mutation in PTCs in a clinical setting.

Citations

Citations to this article as recorded by  
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    Joonseon Park, Solji An, Kwangsoon Kim, Jeong Soo Kim, Chan Kwon Jung, Ja Seong Bae
    Scientific Reports.2024;[Epub]     CrossRef
  • BRAFV600E Positivity-Dependent Effect of Age on Papillary Thyroid Cancer Recurrence Risk
    Joonseon Park, Solji An, Kwangsoon Kim, Ja Seong Bae, Jeong Soo Kim
    Cancers.2023; 15(22): 5395.     CrossRef
  • TROP-2 and 5hmC expression in follicular-patterned thyroid neoplasm emphasizing tiny well-formed papillae
    Jae Yeon Seok, Xuemo Fan
    Annals of Diagnostic Pathology.2022; 57: 151903.     CrossRef
  • Null Association between BRAF V600E Mutation and Tumor Recurrence in Patients with Papillary Thyroid Microcarcinoma in South Korea
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    International Journal of Thyroidology.2021; 14(2): 135.     CrossRef
  • Evaluation of the expression levels of BRAFV600E mRNA in primary tumors of thyroid cancer using an ultrasensitive mutation assay
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    BMC Cancer.2020;[Epub]     CrossRef
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    Irene Vanni, Enrica Teresa Tanda, Francesco Spagnolo, Virginia Andreotti, William Bruno, Paola Ghiorzo
    Frontiers in Molecular Biosciences.2020;[Epub]     CrossRef
  • Highly prevalent BRAF V600E and low-frequency TERT promoter mutations underlie papillary thyroid carcinoma in Koreans
    Sue Youn Kim, Taeeun Kim, Kwangsoon Kim, Ja Seong Bae, Jeong Soo Kim, Chan Kwon Jung
    Journal of Pathology and Translational Medicine.2020; 54(4): 310.     CrossRef
  • Molecular pathology of thyroid tumours of follicular cells: a review of genetic alterations and their clinicopathological relevance
    Giorgia Acquaviva, Michela Visani, Andrea Repaci, Kerry J Rhoden, Dario de Biase, Annalisa Pession, Tallini Giovanni
    Histopathology.2018; 72(1): 6.     CrossRef
  • Development of ultra-short PCR assay to reveal BRAF V600 mutation status in Thai colorectal cancer tissues
    Nunthawut Chat-Uthai, Pichpisith Vejvisithsakul, Sutthirat Udommethaporn, Puttarakun Meesiri, Chetiya Danthanawanit, Yannawan Wongchai, Chinachote Teerapakpinyo, Shanop Shuangshoti, Naravat Poungvarin, John Souglakos
    PLOS ONE.2018; 13(6): e0198795.     CrossRef
  • T-blocker: a simple and robust probe-free quantitative PCR assay to detect somatic mutations down to 0.1% frequency
    Hanyoup Kim, Aaron E Ruby, Harini G Shandilya, Arvind K Virmani, Nandita Rahman, Christina M Strange, Jarkko Huuskonen
    BioTechniques.2018; 65(4): 205.     CrossRef
  • The usefulness of a novel fully automated PCR-based Idylla test for detection of the BRAF V600E mutation in thyroid tissue: comparison with PNA-clamping PCR, real-time PCR and pyrosequencing
    Min-Kyung Yeo, Min-Kyu Jung, Su-Yel Lee, Yong-Moon Lee, Gang Min Hur, Jin-Man Kim
    Journal of Clinical Pathology.2017; 70(3): 260.     CrossRef
  • KRAS mutation analysis of washing fluid from endoscopic ultrasound-guided fine needle aspiration improves cytologic diagnosis of pancreatic ductal adenocarcinoma
    Joo Kyung Park, Yoon Jung Lee, Jong Kyun Lee, Kyu Taek Lee, Yoon-La Choi, Kwang Hyuck Lee
    Oncotarget.2017; 8(2): 3519.     CrossRef
  • Application of Peptide Nucleic Acid-based Assays Toward Detection of Somatic Mosaicism
    Christopher S Hong, Chunzhang Yang, Zhengping Zhuang
    Molecular Therapy - Nucleic Acids.2016; 5: e314.     CrossRef
  • Prediction Table and Nomogram as Tools for Diagnosis of Papillary Thyroid Carcinoma
    Seo Ki Kim, Jun Ho Lee, Jung-Woo Woo, Inhye Park, Jun-Ho Choe, Jung-Han Kim, Jee Soo Kim
    Medicine.2015; 94(21): e760.     CrossRef
  • The association between BRAF V600E mutation and pathological features in PTC
    Xin Liu, Kangkang Yan, Xuejun Lin, Longyu Zhao, Wenxiu An, Chunpeng Wang, Xiaodong Liu
    European Archives of Oto-Rhino-Laryngology.2014; 271(11): 3041.     CrossRef
  • Recurrent Thyroid Papillary Carcinoma in Children Under Ten Years Old: Report of Two Cases and Literature Review
    Byeong-Joo Noh, Ji-Youn Sung, Youn-Wha Kim, Yong-Koo Park
    Korean Journal of Pathology.2014; 48(4): 297.     CrossRef
  • KRASMutation Detection in Non-small Cell Lung Cancer Using a Peptide Nucleic Acid-Mediated Polymerase Chain Reaction Clamping Method and Comparative Validation with Next-Generation Sequencing
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    Korean Journal of Pathology.2014; 48(2): 100.     CrossRef
  • VE1 immunohistochemistry in pituitary adenomas is not associated with BRAF V600E mutation
    Jan Sperveslage, Midea Gierke, David Capper, Jürgen Honegger, Bence Sipos, Rudi Beschorner, Jens Schittenhelm
    Acta Neuropathologica.2013; 125(6): 911.     CrossRef
  • BRAF Mutations in Iranian Patients with Papillary Thyroid Carcinoma
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J Pathol Transl Med : Journal of Pathology and Translational Medicine